[ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#Head", "@graph": [ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI", "http://www.nanopub.org/nschema#hasAssertion": [ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance": [ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo": [ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#pubInfo" } ], "@type": [ "http://www.nanopub.org/nschema#Nanopublication" ] } ] }, { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#pubInfo", "@graph": [ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#assertion", "http://www.w3.org/ns/prov#wasDerivedFrom": [ { "@id": "https://hypothes.is/a/2ytMnollEeui9A8sPwkbMQ" }, { "@id": "https://hypothes.is/a/EzDU7IlmEeu2oL91vbiduA" }, { "@id": "https://hypothes.is/a/_pAwbollEeuy66P1BM98tQ" }, { "@id": "https://hypothes.is/a/iZMZqoj0Eeu-3cOIMEMS4A" } ] }, { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#sig", "http://purl.org/nanopub/x/hasAlgorithm": [ { "@value": "RSA" } ], "http://purl.org/nanopub/x/hasPublicKey": [ { "@value": "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" } ], "http://purl.org/nanopub/x/hasSignature": [ { "@value": "B9c4v8WOACEdTHkx+ll+rPz5wityciZo6AwUxxevJUJxxXq5ZJDkCtDrjDU4nqyKoMQrEHM42l+ztC7dyo5D8K2CUNo4+fk2ajbc818NJ7ijElfN3zWm1y4zfIFkImRZqe76tjm+2eYUD+9dQXNVOu1/962EoyIzhqbk7TuHZ+Y=" } ], "http://purl.org/nanopub/x/hasSignatureTarget": [ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI" } ] }, { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI", "http://purl.org/nanopub/x/introduces": [ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#step" } ], "http://www.w3.org/ns/prov#generatedAtTime": [ { "@value": "2021-03-20T05:38:29.271453", "@type": "http://www.w3.org/2001/XMLSchema#dateTime" } ], "http://www.w3.org/ns/prov#wasAttributedTo": [ { "@id": "https://hypothes.is/users/iannotate" } ] } ] }, { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#assertion", "@graph": [ { "@id": "http://purl.obolibrary.org/obo/CHEBI_8984", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "10% SDS solution" } ] }, { "@id": "http://purl.obolibrary.org/obo/OBI_0400155", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "water bath" } ] }, { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#_1", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "10 mg/ml Proteinase K solution" } ] }, { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#step", "http://purl.org/dc/elements/1.1/description": [ { "@value": "Add 1 ml of 10% SDS solution (final concentration, ~1%) and 20 µl of 10 mg/ml Proteinase K solution (final concentration 20 µg/ml). Incubate for 2 to 4 h at 60 °C in a hot water bath." } ], "@type": [ "http://purl.org/net/p-plan#Step" ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed": [ { "@id": "http://purl.obolibrary.org/obo/OBI_0400155" } ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed": [ { "@id": "http://purl.obolibrary.org/obo/CHEBI_8984" }, { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#_1" } ] } ] }, { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#provenance", "@graph": [ { "@id": "http://purl.org/np/RAja3xRrvfm-JzDWWT_6jYJxO4lshPlLjtS1YREPsqjoI#assertion", "http://purl.org/pav/createdWith": [ { "@id": "https://nanotate.bitsfetch.com" } ], "http://www.w3.org/ns/prov#generatedAtTime": [ { "@value": "2021-03-20T05:38:29.271453", "@type": "http://www.w3.org/2001/XMLSchema#dateTime" } ], "http://www.w3.org/ns/prov#wasAttributedTo": [ { "@id": "https://hypothes.is/users/iannotate" } ] } ] } ]