[ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#pubInfo", "@graph": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#assertion", "http://www.w3.org/ns/prov#wasDerivedFrom": [ { "@id": "https://hypothes.is/a/StSUVnJLEeuj40cSjvHiHA" }, { "@id": "https://hypothes.is/a/deJajnJLEeu9FNuSHOkqmw" } ] }, { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#sig", "http://purl.org/nanopub/x/hasAlgorithm": [ { "@value": "RSA" } ], "http://purl.org/nanopub/x/hasPublicKey": [ { "@value": "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" } ], "http://purl.org/nanopub/x/hasSignature": [ { "@value": "R5SR6FOgnMCKNbbTZs8dnRV+QvjH6PLBXELVshpOw3RLlqtOIXpj9AsIvz4C3qA43+ZPFyFwUcG+zTaM7jzlvn6jVzu4EiUvA4auc/gbt8J+Kt9BMa9/A+5Bu3hzz017V1ynjjnvSnmY0ZM9+uvmH/vpMyE74wMgI2yU5cqvf/o=" } ], "http://purl.org/nanopub/x/hasSignatureTarget": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4" } ] }, { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4", "http://purl.org/nanopub/x/introduces": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#step" } ], "http://www.w3.org/ns/prov#generatedAtTime": [ { "@value": "2021-03-10T10:17:44.515088", "@type": "http://www.w3.org/2001/XMLSchema#dateTime" } ], "http://www.w3.org/ns/prov#wasAttributedTo": [ { "@id": "https://hypothes.is/users/alejandra.sandoval" } ] } ] }, { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#assertion", "@graph": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#_1", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "solubilized nucleic acid" } ] }, { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#_2", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "solubilized nucleic acid" } ] }, { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#step", "http://purl.org/dc/elements/1.1/description": [ { "@value": "After incubating at room temperature for a few minutes, keep the solubilized nucleic acid in −20˚C for a short time storage or in −80˚C for a longtime storage. Figure 1 shows a schematic model of all the DNA and RNA isolation steps of this procedure." } ], "http://purl.org/net/SMARTprotocol#hasExperimentalOutput": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#_1" } ], "@type": [ "http://purl.org/net/p-plan#Step" ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#_2" } ] } ] }, { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#Head", "@graph": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4", "http://www.nanopub.org/nschema#hasAssertion": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#pubInfo" } ], "@type": [ "http://www.nanopub.org/nschema#Nanopublication" ] } ] }, { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#provenance", "@graph": [ { "@id": "http://purl.org/np/RAft0TBkyd36_sDa0cAhIEBlaAR71Aicotgr53DfmR0_4#assertion", "http://purl.org/pav/createdWith": [ { "@id": "https://nanotate.bitsfetch.com" } ], "http://www.w3.org/ns/prov#generatedAtTime": [ { "@value": "2021-03-10T10:17:44.515088", "@type": "http://www.w3.org/2001/XMLSchema#dateTime" } ], "http://www.w3.org/ns/prov#wasAttributedTo": [ { "@id": "https://hypothes.is/users/alejandra.sandoval" } ] } ] } ]