Nanopublication

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ID

http://purl.org/np/RAYehDuYU12FcErOeUL91Sm9D92FV23Vh4Bn4bmtC7cYE

Formats

.trig | .trig.txt | .jelly | .jelly.txt | .jsonld | .jsonld.txt | .nq | .nq.txt | .xml | .xml.txt

Content

@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix this: <http://purl.org/np/RAYehDuYU12FcErOeUL91Sm9D92FV23Vh4Bn4bmtC7cYE> .
@prefix sub: <http://purl.org/np/RAYehDuYU12FcErOeUL91Sm9D92FV23Vh4Bn4bmtC7cYE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: a np:Nanopublication;
    np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo .
}

sub:assertion {
  sub:_1 rdfs:label "clear supernatant" .
  
  sub:_2 rdfs:label "clear supernatant" .
  
  sub:_3 rdfs:label "chloroform: isoamyl alcohol" .
  
  sub:_4 rdfs:label "RNAse A" .
  
  sub:_5 rdfs:label "5 M NaCl " .
  
  sub:step a pplan:Step;
    dce:description "After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions";
    sp:hasExperimentalOutput sub:_1;
    bs:bioSampleUsed sub:_2;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed sub:_3, sub:_4, sub:_5 .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T10:51:38.348759"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:8tPhFoFLEeugr5u2BPA1Kw, authority:GghLeIFMEeu23ZtkPfVRMA,
      authority:P5lxUIFMEeuVocP4Xt_PMA, authority:ZrwoGIFMEeu00ofuLN-6KA, authority:hQCEroFMEeuvmA-YJowmfw,
      authority:p826BoFMEeuUHBMr8wtiOw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "f0RlLZxhjVratyK9HwJAFBjprtgMh+s1m9+GW+jKAA8WPDWP5lA8bJum+UJvgUKLeNKp2TpxFizIWPmFxu6MJOHFR04DL+Z/C35T5ZOKWK4Kjg1lwIFLvo4EewgcKDt3yvqwLM/azBLA/36DJyW+2UpE6P50Fmh/Bb0tv52N+Ug=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T10:51:38.348759"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}