[ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#Head", "@graph": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA", "http://www.nanopub.org/nschema#hasAssertion": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#pubInfo" } ], "@type": [ "http://www.nanopub.org/nschema#Nanopublication" ] } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#pubInfo", "@graph": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#assertion", "http://www.w3.org/ns/prov#wasDerivedFrom": [ { "@id": "https://hypothes.is/a/IMGwSnbiEeuyhtu9n7srbg" }, { "@id": "https://hypothes.is/a/NDLvkHbiEeuvGws9CpXYEg" }, { "@id": "https://hypothes.is/a/RxAaSHbiEeuf9Qs7D-7mbQ" }, { "@id": "https://hypothes.is/a/W5TnvnbiEeu9zJfZKE5SbA" }, { "@id": "https://hypothes.is/a/cQA86HbiEeuDoKPmfzScPg" } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#sig", "http://purl.org/nanopub/x/hasAlgorithm": [ { "@value": "RSA" } ], "http://purl.org/nanopub/x/hasPublicKey": [ { "@value": "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" } ], "http://purl.org/nanopub/x/hasSignature": [ { "@value": "AHJ5Yc8Tzak1K5M6ymjq/Lx8OO2o5lM3p94CzafH5Ni0VOBC/azC+NBC97XRdzkbhQZ4MweCnsIMI6wZLEIdTPaziFpTvDx6EuDyaonhR3iTRXpGg1mHvgoaUCIyt3vudun5cVc0SIyFfCdUlKtB5OtwrVgMjXRJt+ug1mdMzh4=" } ], "http://purl.org/nanopub/x/hasSignatureTarget": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA" } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA", "http://purl.org/nanopub/x/introduces": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#step" } ], "http://www.w3.org/ns/prov#generatedAtTime": [ { "@value": "2021-02-24T15:55:41.254735", "@type": "http://www.w3.org/2001/XMLSchema#dateTime" } ], "http://www.w3.org/ns/prov#wasAttributedTo": [ { "@id": "https://hypothes.is/users/zuluaga.lizzz" } ] } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#assertion", "@graph": [ { "@id": "http://purl.obolibrary.org/obo/CHEBI_16991", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "DNA" } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#_1", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "pellet" } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#_2", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "pellet" } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#_3", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "nuclease free water" } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#_4", "http://www.w3.org/2000/01/rdf-schema#label": [ { "@value": "TE buffer" } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#step", "http://purl.org/dc/elements/1.1/description": [ { "@value": "Dissolve the dry pellet in nuclease free water or TE buffer (Ph 8.0). Store DNA at 4 º celcius or -20 º celcius ." } ], "http://purl.org/net/SMARTprotocol#hasExperimentalOutput": [ { "@id": "http://purl.obolibrary.org/obo/CHEBI_16991" }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#_1" } ], "@type": [ "http://purl.org/net/p-plan#Step" ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed": [ { "@id": "http://purl.obolibrary.org/obo/CHEBI_16991" }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#_2" } ], "https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#_3" }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#_4" } ] } ] }, { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#provenance", "@graph": [ { "@id": "http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#assertion", "http://purl.org/pav/createdWith": [ { "@id": "https://nanotate.bitsfetch.com" } ], "http://www.w3.org/ns/prov#generatedAtTime": [ { "@value": "2021-02-24T15:55:41.254735", "@type": "http://www.w3.org/2001/XMLSchema#dateTime" } ], "http://www.w3.org/ns/prov#wasAttributedTo": [ { "@id": "https://hypothes.is/users/zuluaga.lizzz" } ] } ] } ]